{"id":50,"date":"2016-05-22T08:40:54","date_gmt":"2016-05-22T08:40:54","guid":{"rendered":"http:\/\/ecobioinfo.com\/bio\/?page_id=48"},"modified":"2017-01-09T21:10:13","modified_gmt":"2017-01-09T12:10:13","slug":"%e7%92%b0%e5%a2%83%e5%be%ae%e7%94%9f%e7%89%a9","status":"publish","type":"page","link":"http:\/\/www.ecobioinfo.com\/?page_id=50","title":{"rendered":"\u74b0\u5883\u4e2d\u306e\u5fae\u751f\u7269\u306e\u7814\u7a76"},"content":{"rendered":"<p><span style=\"color: blue;\"><b>\u74b0\u5883\u4e2d\u306e\u5fae\u751f\u7269\u306e\u7814\u7a76<\/b><br \/>\n<\/span><br \/>\n\u5fae\u751f\u7269\u306e\u3046\u3061\u4eba\u985e\u304c\u628a\u63e1\u3057\u3066\u3044\u308b\u3082\u306e\u306f\u5168\u4f53\u306e\uff12\u5272\u7a0b\u5ea6\u3060\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u69d8\u3005\u306a\u74b0\u5883\u4e2d\u306b\u751f\u606f\u3059\u308b\u672a\u77e5\u306e\u5fae\u751f\u7269\u306e\u4e2d\u306b\u306f\u3001\u4eba\u985e\u306b\u3068\u3063\u3066\u6709\u7528\u306a\u751f\u7269\u3084\u3001\u9006\u306b\u5371\u967a\u306a\u751f\u7269\u304c\u5b58\u5728\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u3088\u3046\u306a\u69d8\u3005\u306a\u74b0\u5883\u4e2d\u306b\u751f\u606f\u3059\u308b\u5fae\u751f\u7269\u3092\u8abf\u67fb\u7814\u7a76\u3059\u308b\u3053\u3068\u306f\u74b0\u5883\u554f\u984c\u306e\u89e3\u6c7a\u3084\u8cc7\u6e90\u56de\u53ce\u3001\u75c5\u539f\u5fae\u751f\u7269\u306b\u3088\u308b\u5371\u967a\u306e\u56de\u907f\u306a\u3069\u306b\u95a2\u308f\u308b\u4eca\u5f8c\u306e\u91cd\u8981\u306a\u30c6\u30fc\u30de\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<td>1 \u74b0\u5883\u4e2d\u304b\u3089\u5fae\u751f\u7269\u3092\u5358\u96e2\u57f9\u990a\u3059\u308b<\/p>\n<table>\n<tbody>\n<tr>\n<td>\u74b0\u5883\u4e2d\u306b\u751f\u606f\u3059\u308b\u5fae\u751f\u7269\u3092\u8abf\u3079\u308b\u5834\u5408\u3001\u57f9\u990a\u65b9\u6cd5\u304c\u3059\u3067\u306b\u77e5\u3089\u308c\u3066\u3044\u308b\u5fae\u751f\u7269\u3067\u306f\u7279\u5b9a\u306e\u751f\u7269\u3092\u9078\u629e\u7684\u306b\u57f9\u990a\u3059\u308b\u57f9\u5730\u304c\u3064\u304b\u308f\u308c\u307e\u3059\u3002<br \/>\n\u4f8b\u3048\u3070\u3001\u5ddd\u306e\u6c34\u306e\u5927\u8178\u83cc\u7fa4\u691c\u67fb\u3092\u884c\u3046\u5834\u5408\u306f\u5927\u8178\u83cc\u7fa4\u691c\u67fb\u7528\u306e\u57f9\u5730\u304c\u4f7f\u308f\u308c\u307e\u3059\u3002<br \/>\n\u672a\u77e5\u306e\u5fae\u751f\u7269\u3092\u542b\u3080\u74b0\u5883\u4e2d\u306e\u5fae\u751f\u7269\u3092\u7d14\u7c8b\u57f9\u990a\u3059\u308b\u306b\u306f\u3001\u4e00\u822c\u7684\u306b\u751f\u606f\u74b0\u5883\u306b\u8fd1\u3044\u6210\u5206\u306e\u57f9\u5730\u3092\u4f7f\u3063\u3066\u57f9\u990a\u3057\u3066\u5897\u3048\u305f\u5fae\u751f\u7269\u3092\u5206\u96e2\u3057\u3066\u57f9\u990a\u3057\u3066\u5206\u6790\u3059\u308b\u65b9\u6cd5\u304c\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u3001\u74b0\u5883\u4e2d\u304b\u3089\u5fae\u751f\u7269\u3092\u5358\u96e2\u3057\u3066\u7d14\u7c8b\u57f9\u990a\u3068\u3059\u308b\u306b\u306f\u4e00\u822c\u7684\u306b\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u65b9\u6cd5\u3067\u884c\u3044\u307e\u3059\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td>\n<ol>\n<li>\u74b0\u5883\u4e2d\u304b\u3089\u30b5\u30f3\u30d7\u30eb\uff08\u6c34\u3001\u6ce5\u3001\u306a\u3069\uff09\u3092\u63a1\u308b\u3002<\/li>\n<li>\u30b5\u30f3\u30d7\u30eb\u304c\u6ce5\u306e\u3088\u3046\u306a\u534a\u56fa\u5f62\u7269\u306e\u5834\u5408\u3084\u5fae\u751f\u7269\u304c\u304b\u305f\u307e\u3063\u3066\u3044\u308b\u5834\u5408\u306f\u7269\u7406\u7684\u306b\u5206\u6563\u3055\u305b\u308b\u3002<\/li>\n<li>\u30b5\u30f3\u30d7\u30eb\u3092\u6bb5\u968e\u7684\u306b\u5e0c\u91c8\u3059\u308b\uff08\u4f8b\u3048\u3070\uff11\uff10\u500d\u3001\uff11\uff10\uff10\u500d\u3001\u3001\u3001\uff09\u3002<\/li>\n<li>\u5e0c\u91c8\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u305d\u308c\u305e\u308c\u4e00\u5b9a\u91cf\u53d6\u308a\u57f9\u990a\u3059\u308b\u3002\u5fae\u751f\u7269\u3092\u57f9\u990a\u3059\u308b\u57f9\u5730\u306f\u3001\u57f9\u990a\u306b\u5fc5\u8981\u306a\u6804\u990a\u3092\u5bd2\u5929\u3067\u56fa\u3081\u305f\u5e73\u677f\u57f9\u5730\u304c\u3064\u304b\u308f\u308c\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002<br \/>\n\u5e73\u677f\u57f9\u5730\u306e\u4e0a\u3067\u5897\u3048\u305f\u5fae\u751f\u7269\u306f\u5e73\u677f\u57f9\u5730\u306e\u4e0a\u3067\u30b3\u30ed\u30cb\u30fc\uff08\u5fae\u751f\u7269\u306e\u584a\uff09\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u3000\u5145\u5206\u306b\u5e0c\u91c8\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u5e73\u677f\u57f9\u5730\u4e0a\u306e\u30b3\u30ed\u30cb\u30fc\u306e\u5143\u306f\u4e00\u3064\u306e\u5fae\u751f\u7269\u304b\u3089\u7d30\u80de\u5206\u88c2\u3067\u5897\u3048\u305f\u3082\u306e\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/li>\n<li>\u5145\u5206\u306b\u5e0c\u91c8\u3055\u308c\u3066\u3044\u308b\u57f9\u5730\u3067\u5897\u3048\u305f\u5fae\u751f\u7269\u3092\u305d\u308c\u305e\u308c\u53d6\u308a\u3001\u518d\u3073\u5e0c\u91c8\u3068\u57f9\u990a(\u4f8b\u3048\u3070\u3001\u5e73\u677f\u57f9\u5730\u306e\u5834\u5408\u3001\u8584\u304f\u5857\u308a\u76f4\u3057\u3066\u57f9\u990a)\u3092\u7e70\u308a\u8fd4\u3059\u3002<\/li>\n<li>\u4e00\u3064\u306e\u5fae\u751f\u7269\u304b\u3089\u5897\u3048\u305f\u7d14\u7c8b\u57f9\u990a\u306b\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u3002\u5fae\u751f\u7269\u306e\u7a2e\u985e\u3068\u7d14\u7c8b\u57f9\u990a\u306b\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u65b9\u6cd5\u306f\u305d\u308c\u305e\u308c\u306e\u5fae\u751f\u7269\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002\u8fd1\u5e74\u3067\u306f\u907a\u4f1d\u5b50\u3092\u62bd\u51fa\u30fb\u5206\u96e2\u30fb\u5897\u5e45\u3057\u3066\uff24\uff2e\uff21\u5869\u57fa\u914d\u5217\u3092\u8abf\u3079\u308b\u65b9\u6cd5\u304c\u666e\u53ca\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ol>\n<\/td>\n<td align=\"center\"><img decoding=\"async\" src=\"http:\/\/ecobioinfo.com\/usr\/images\/plate01.jpg\" alt=\"\" \/><span style=\"color: blue;\">\u56f32-1\uff1a\u5e73\u677f\u5bd2\u5929\u57f9\u5730<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>2 \u5206\u5b50\u751f\u7269\u5b66\u7684\u624b\u6cd5<\/p>\n<table>\n<tbody>\n<tr>\n<td>\u69d8\u3005\u306a\u74b0\u5883\u4e2d\u306b\u751f\u606f\u3057\u3066\u3044\u308b\u5fae\u751f\u7269\u306b\u306f\u62bd\u51fa\u30fb\u57f9\u990a\u304c\u56f0\u96e3\u306a\u3082\u306e\u304c\u591a\u304f\u3044\u307e\u3059\u304c\u3001<br \/>\n\u76f4\u63a5\u306e\u89b3\u5bdf\u304c\u56f0\u96e3\u306a\u5fae\u751f\u7269\u3067\u3082\u5206\u5b50\u751f\u7269\u5b66\u7684\u624b\u6cd5\u306a\u3069\u3092\u7528\u3044\u305f\u9593\u63a5\u7684\u306a\u624b\u6bb5\u306b\u3088\u3063\u3066\u3042\u308b\u7a0b\u5ea6\u306e\u60c5\u5831\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<br \/>\n\u8fd1\u5e74\u3067\u306f\u5fae\u751f\u7269\u306e\u691c\u51fa\u306b\u907a\u4f1d\u5b50\u3092\u5229\u7528\u3057\u305f\u624b\u6cd5\u304c\u63a1\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u591a\u304f\u306a\u308a\u307e\u3057\u305f\u3002<br \/>\n\u7279\u306b\u5f62\u614b\u7684\u7279\u5fb4\u306e\u4e4f\u3057\u3044\u7d30\u83cc\u3067\u306f\u300116SrDNA\u3068\u547c\u3070\u308c\u308b\u907a\u4f1d\u5b50\u3092\u7528\u3044\u3066\u7d30\u83cc\u306e\u5b58\u5728\u3092\u691c\u51fa\u3059\u308b\u624b\u6cd5\u304c\u5404\u5206\u91ce\u3067\u5e83\u304f\u7528\u3044\u3089\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u3066\u304d\u307e\u3057\u305f\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr>\n<td>2-1 \uff24\uff2e\uff21\uff08\uff32\uff2e\uff21\uff09\u62bd\u51fa\u3068\u907a\u4f1d\u5b50\u7cfb\u7d71\u89e3\u6790<\/p>\n<table>\n<tbody>\n<tr>\n<td>\u540c\u3058\u50cd\u304d\u3092\u3059\u308b\u907a\u4f1d\u5b50\u3067\u3082\uff24\uff2e\uff21\u5869\u57fa\u914d\u5217\u306b\u306f\u5909\u7570\u304c\u3042\u308a\u307e\u3059\u3002\u9055\u3046\u751f\u7269\u540c\u58eb\u3067\u3082\u3001\u751f\u7269\u5171\u901a\u3067\u540c\u3058\u50cd\u304d\u3092\u3059\u308b\u907a\u4f1d\u5b50\u306e\uff24\uff2e\uff21\u3084\uff32\uff2e\uff21\u306e\u5869\u57fa\u914d\u5217\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u751f\u7269\u306e\u9032\u5316\u7684\u306a\u8fd1\u7e01\u95a2\u4fc2\u3092\u63a8\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u3053\u306e\u907a\u4f1d\u5b50\u30ec\u30d9\u30eb\u3067\u306e\u9032\u5316\u7684\u306a\u8fd1\u7e01\u95a2\u4fc2\u3092\u6c42\u3081\u308b\u3053\u3068\u3092\u907a\u4f1d\u5b50\u7cfb\u7d71\u89e3\u6790\u3001\u305d\u308c\u3092\u56f3\u306b\u3057\u305f\u3082\u306e\u3092\u907a\u4f1d\u5b50\u7cfb\u7d71\u6a39\u3068\u547c\u3073\u307e\u3059\u3002<br \/>\n\u7d30\u83cc\u306e\u5834\u5408\u306f\u300116SrDNA\u3068\u547c\u3070\u308c\u308b\u907a\u4f1d\u5b50\u306f\u540c\u3058\u7a2e\u985e\u306e\u7d30\u83cc\u3067\u306f\uff24\uff2e\uff21\u306e\u5909\u7570\u304c\u5c11\u306a\u3044\u306e\u3067\u7a2e\u985e\u3092\u5224\u5225\u3059\u308b\u306e\u306b\u3082\u4f7f\u308f\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<br \/>\n\u81ea\u7136\u74b0\u5883\u4e2d\u306b\u751f\u606f\u3059\u308b\u7d30\u83cc\u304b\u3089\u5206\u5b50\u751f\u7269\u7684\u624b\u6cd5\u306b\u3088\u3063\u3066\uff24\uff2e\uff21\u306e\u307f\u3092\u62bd\u51fa\u3001\uff24\uff2e\uff21\u5869\u57fa\u914d\u5217\u306e\u9055\u3044\u306b\u3088\u3063\u3066\u5206\u96e2\u3057\u3066\u3001\u305d\u308c\u305e\u308c\u306e\uff24\uff2e\uff21\u306e\u907a\u4f1d\u5b50\u7cfb\u7d71\u3092\u89e3\u6790\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3069\u306e\u3088\u3046\u306a\u7d30\u83cc\u304c\u751f\u606f\u3057\u3066\u3044\u308b\u304b\u3092\u8abf\u67fb\u3059\u308b\u3053\u3068\u3082\u884c\u308f\u308c\u3066\u3044\u307e\u3059\u3002<a href=\"http:\/\/www.jseb.jp\/jeb\/jeb.html\" target=\"_blank\">\u53c2\u8003\uff1a\u74b0\u5883\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u5b66\u4f1a\uff08\u30ea\u30f3\u30af\uff09\u3000(\u7dcf\u8aac)\u571f\u58cc\u304b\u3089\u306eDNA\u62bd\u51fa\u6cd5<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr>\n<td>2-2 \u7d30\u83cc\u3092\uff24\uff2e\uff21\u3067\u67d3\u3081\u308b\uff08Fluorescence In Situ Hybridization\uff09<\/p>\n<table>\n<tbody>\n<tr>\n<td align=\"left\" valign=\"top\">\u77ed\u3044\uff24\uff2e\uff21\u306b\u86cd\u5149\u7269\u8cea\u3092\u7d50\u5408\u3055\u305b\u305f\u300c\u30d7\u30ed\u30fc\u30d6\u300d\u3092\u7d30\u83cc\u5185\u306e\uff32\uff2e\uff21\u3068\u7d50\u5408\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u7d30\u83cc\u3092\u691c\u51fa\u3059\u308b\u65b9\u6cd5\u3082\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u65b9\u6cd5\u306f\uff26\uff29\uff33\uff28\uff08Fluorescence In Situ Hybridization\uff09\u3068\u547c\u3070\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u4f8b\u3048\u3070\u3001\u6ce5\u306e\u4e2d\u304b\u3089\u62bd\u51fa\u3057\u305f\u672a\u77e5\u306e\u7d30\u83cc\u306e\uff24\uff2e\uff21\u304b\u3089\u30d7\u30ed\u30fc\u30d6\u3092\u8a2d\u8a08\u3057\u3066\uff26\uff29\uff33\uff28\u6cd5\u3092\u9069\u7528\u3059\u308b\u3068\u3001\u305d\u306e\uff24\uff2e\uff21\u3092\u3082\u3064\u7d30\u83cc\u304c\u3069\u306e\u3088\u3046\u306a\u5f62\u3067\u3001\u3069\u306e\u7a0b\u5ea6\u751f\u606f\u3057\u3066\u3044\u308b\u304b\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/td>\n<td><img decoding=\"async\" src=\"http:\/\/ecobioinfo.com\/usr\/images\/pao-fish01.jpg\" alt=\"\" \/><span style=\"color: blue;\">\u9855\u5fae\u93e1\u5199\u771f2-1\uff1a\uff26\uff29\uff33\uff28\u6cd5\u3067\u7279\u5b9a\u306e\u7d30\u83cc\u3092\u691c\u51fa<\/span><\/p>\n<p>\u5199\u771f\u306e\u8d64\u8272\u304c\u76ee\u7684\u306e\u7d30\u83cc\u3001\u7dd1\u8272\u306f\u305d\u306e\u4ed6\u306e\u7d30\u83cc<\/td>\n<td>\n<table>\n<tbody>\n<tr>\n<td><img decoding=\"async\" src=\"http:\/\/ecobioinfo.com\/usr\/images\/pao-fish02.jpg\" alt=\"\" \/><\/td>\n<td><img decoding=\"async\" src=\"http:\/\/ecobioinfo.com\/usr\/images\/pao-dapi01.jpg\" alt=\"\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"color: blue;\">\u9855\u5fae\u93e1\u5199\u771f2-2\uff1a\u57f9\u990a\u3067\u304d\u306a\u3044\u30dd\u30ea\u30ea\u30f3\u9178\u84c4\u7a4d\u83cc\u3092\uff26\uff29\uff33\uff28\u6cd5\u3067\u691c\u51fa<\/span><\/p>\n<p>\u5de6\u306e\u8d64\u8272\u304c\uff26\uff29\uff33\uff28\u6cd5\u306e\u7d50\u679c\u3001\u53f3\u306f\u540c\u3058\u30d7\u30ec\u30d1\u30e9\u30fc\u30c8\u3067\u30dd\u30ea\u30ea\u30f3\u9178\u3092\u67d3\u8272\u51e6\u7406\uff08\u9ec4\u8272\u306e\u90e8\u5206\uff09\u3002\u5de6\u53f3\u306e\u7d50\u679c\u304b\u3089\u3053\u306e\u7d30\u83cc\u304c\u30dd\u30ea\u30ea\u30f3\u9178\u3092\u542b\u3093\u3067\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\uff1c\uff1c\u53c2\u8003\u6587\u732e\uff1e\uff1e<\/p>\n<p>2.Polyphasic approaches to the identification of predominant polyphosphate-accumulating organisms in a laboratory-scale anaerobic\/aerobic activated sludge system \/<br \/>\nOnda,S. et al. \/<br \/>\nThe Journal of General and Applied Microbiology, Vol. 48 (2002) No. 1 pp.43-54<\/p>\n<p>3.Isolation and characterization of a Gram-positive polyphosphate-accumulating bacterium \/<br \/>\nOnda,S. and Takii,S. \/<br \/>\nThe Journal of General and Applied Microbiology, Vol. 48 (2002) No. 3 pp.125-133<\/p>\n","protected":false},"excerpt":{"rendered":"\u74b0\u5883\u4e2d\u306e\u5fae\u751f\u7269\u306e\u7814\u7a76 \u5fae\u751f\u7269\u306e\u3046\u3061\u4eba\u985e\u304c\u628a\u63e1\u3057\u3066\u3044\u308b\u3082\u306e\u306f\u5168\u4f53\u306e\uff12\u5272\u7a0b\u5ea6\u3060\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u69d8\u3005\u306a\u74b0\u5883\u4e2d\u306b\u751f\u606f\u3059\u308b\u672a\u77e5\u306e\u5fae\u751f\u7269\u306e\u4e2d\u306b\u306f\u3001\u4eba\u985e\u306b\u3068\u3063\u3066\u6709\u7528\u306a\u751f\u7269\u3084\u3001\u9006\u306b\u5371\u967a\u306a\u751f\u7269\u304c\u5b58\u5728\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u3088\u3046\u306a\u69d8\u3005\u306a 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