{"id":1498,"date":"2022-06-08T23:15:08","date_gmt":"2022-06-08T14:15:08","guid":{"rendered":"http:\/\/www.ecobioinfo.com\/?p=1498"},"modified":"2022-06-09T22:24:49","modified_gmt":"2022-06-09T13:24:49","slug":"%ef%bd%81%ef%bd%89%e8%aa%b2%e9%a1%8c%ef%bc%92%ef%bc%9a%ef%bd%81%ef%bd%89%e3%81%a7%e7%94%9f%e8%8f%8c%e6%95%b0%e3%82%92%e3%82%ab%e3%82%a6%e3%83%b3%e3%83%88%ef%bc%88%ef%bc%92%ef%bc%89","status":"publish","type":"post","link":"http:\/\/www.ecobioinfo.com\/?p=1498","title":{"rendered":"\uff21\uff29\u8ab2\u984c\uff12\uff1a\uff21\uff29\u3067\u751f\u83cc\u6570\u3092\u30ab\u30a6\u30f3\u30c8\uff08\uff12\uff09"},"content":{"rendered":"

\uff08\u300c\uff21\uff29\u8ab2\u984c\uff12\uff1a\uff21\uff29\u3067\u751f\u83cc\u6570\u3092\u30ab\u30a6\u30f3\u30c8\uff08\uff11\uff09<\/a>\u300d\u306e\u7d9a\u304d\u3067\u3059\u3002\uff09<\/p>\n

\u307e\u305a\u6bd4\u8f03\u7684\u7c21\u5358\u306b\u3067\u304d\u305d\u3046\u306a\u300c(2) \u65e2\u5b58\u306e\u81ea\u52d5\u30ab\u30a6\u30f3\u30bf\u306e\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u306e\u524d\u51e6\u7406\u3068\u3057\u3066\u30c7\u30a3\u30fc\u30d7\u30e9\u30fc\u30cb\u30f3\u30b0\u306e\u5b66\u7fd2\u7d50\u679c\u3092\u4f7f\u3046\u5834\u5408\u300d\u306b\u3064\u3044\u3066\u8003\u3048\u308b\u3053\u3068\u306b\u3057\u307e\u3059\u3002<\/p>\n

\u30c7\u30a3\u30fc\u30d7\u30e9\u30fc\u30cb\u30f3\u30b0\u3092\u5229\u7528\u3057\u3066\u5bd2\u5929\u57f9\u5730\u306e\u751f\u83cc\u6570\u3092\u81ea\u52d5\u30ab\u30a6\u30f3\u30c8\u3059\u308b\u4e88\u5b9a\u3067\u3059\u304c\u3001\u305d\u306e\u524d\u306bOpenCV\u3092\u5229\u7528\u3057\u3066\u65e2\u5b58\u306e\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u3067\u3069\u3053\u307e\u3067\u30ab\u30a6\u30f3\u30c8\u3067\u304d\u308b\u304b\u691c\u8a3c\u3059\u308b\u305f\u3081\u306b\u30b5\u30f3\u30d7\u30eb\u30d7\u30ed\u30b0\u30e9\u30e0\u3092\u4f5c\u6210\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n

\u57fa\u672c\u7684\u306b\u306fOpenCV\u306e\u8f2a\u90ed\u691c\u51fa\u6a5f\u80fd findContours \u3092\u5229\u7528\u3057\u3066\u30ab\u30a6\u30f3\u30c8\u3057\u307e\u3059\u3002<\/p>\n

\u30d7\u30ed\u30b0\u30e9\u30e0(\u8a00\u8a9e Python\u3001\u30c6\u30ad\u30b9\u30c8\u30d5\u30a1\u30a4\u30eb UTF-8\uff09<\/p>\n

bactcounter.py<\/a><\/p>\n

\u3010\u89e3\u8aac\u3011<\/p>\n

\u57f9\u5730\u306e\u5199\u771f\u3092\u5165\u529b\u30d5\u30a1\u30a4\u30eb\u3068\u3057\u307e\u3059\u304c\u3001\u30d5\u30a1\u30a4\u30eb\u540d\u306f\u3068\u308a\u3042\u3048\u305a\u4e0b\u8a18\u306e\u3088\u3046\u306b\u30d7\u30ed\u30b0\u30e9\u30e0\u4e2d\u3067\u56fa\u5b9a\u3068\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u30d5\u30a1\u30a4\u30eb\u30d1\u30b9\u3000\u5165\u529b\u30d5\u30a1\u30a4\u30eb\u540d
\nFILE_ORG_IMAGE = ‘data\/counter\/bctimage.jpg’<\/p>\n

\u30d5\u30a1\u30a4\u30eb\u30d1\u30b9\u3000\u51fa\u529b\u30d5\u30a1\u30a4\u30eb\u540d
\nFILE_CNT1_IMAGE = ‘data\/counter\/cntiamge1.jpg’
\nFILE_CNT2_IMAGE = ‘data\/counter\/cntimage2.jpg’<\/p>\n

\u30c6\u30b9\u30c8\u306b\u4f7f\u3063\u305f\u57f9\u5730\u306e\u5199\u771f\u3002<\/p>\n

\"bctimage\"<\/p>\n

\u3053\u306e\u57f9\u5730\u306e\u5199\u771f\u306f\u30c6\u30b9\u30c8\u7528\u306b\u4f5c\u6210\u3057\u305f\u3082\u306e\u3067\u5b9f\u969b\u306b\u57f9\u990a\u3057\u305f\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

\u3053\u306e\u5199\u771f\u306f\u30b9\u30de\u30fc\u30c8\u30d5\u30a9\u30f3\u306e\u30ab\u30e1\u30e9\u3067\u64ae\u5f71\u3057\u305f\u3082\u306e\u3067\u3001\u5c02\u7528\u306e\u8a2d\u5099\u3067\u64ae\u5f71\u3057\u305f\u5199\u771f\u306b\u304f\u3089\u3079\u3066\u4f59\u8a08\u306a\u53cd\u5c04\u3084\u5f71\u306a\u3069\u304c\u6620\u3063\u3066\u3044\u308b\u6761\u4ef6\u306e\u60aa\u3044\u5199\u771f\u3067\u3059\u3002<\/p>\n

\u5b9f\u884c\u3059\u308b\u3068\u57f9\u5730\u306e\u5199\u771f\u3092\u8aad\u307f\u8fbc\u307f\u3001\u30a6\u30a3\u30f3\u30c9\u30a6\u306b\u30b0\u30ec\u30fc\u30b9\u30b1\u30fc\u30eb\u3067\u8868\u793a\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u57f9\u5730\u306e\u7bc4\u56f2\u3068\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u3092\u624b\u52d5\u3067\u8abf\u6574\u3057\u307e\u3059\u3002\u30ad\u30fc\u64cd\u4f5c\u306f\u4ee5\u4e0b\u306e\u3088\u3046\u306b\u9069\u5f53\u306b\u5272\u308a\u632f\u308a\u307e\u3057\u305f\u3002<\/p>\n

\u30fbEnter\u30ad\u30fc\u3067\u6b21\u306e\u51e6\u7406\u3078
\n\u30fbEsc\u30ad\u30fc\u3067\u3084\u308a\u76f4\u3057
\n\u30fb\u57f9\u5730\u7bc4\u56f2\u306e\u5186 ‘o’ = \u5927\u304d\u304f ‘c’ = \u5c0f\u3055\u304f
\n\u30fb\u57f9\u5730\u7bc4\u56f2\u306e\u5186 \u4e2d\u5fc3\u4f4d\u7f6e\u306e\u8abf\u6574\u3000’u’ = \u4e0a ‘d’ = \u4e0b ‘r’ = \u53f3 ‘l’ = \u5de6
\n\u30fb\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8 ‘s’ = \u5f37\u304f ‘w’ = \u5f31\u304f<\/p>\n

Enter\u30ad\u30fc\u3092\u62bc\u3059\u3068\u4e8c\u5024\u5316\u3055\u308c\u305f\u753b\u50cf\u304c\u30a6\u30a3\u30f3\u30c9\u30a6\u306b\u8868\u793a\u3055\u308c\u307e\u3059\u3002<\/p>\n

\"cntiamge1\"<\/p>\n

\u767d\u3044\u9818\u57df\u304c\u62bd\u51fa\u3055\u308c\u3066\u30ab\u30a6\u30f3\u30c8\u3055\u3066\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u5143\u306e\u753b\u50cf\u306b\u3082\u30ab\u30a6\u30f3\u30c8\u5024\u3092\u5165\u308c\u307e\u3057\u305f\u3002<\/p>\n

\"cntimage2\"<\/p>\n

\u30d7\u30ed\u30b0\u30e9\u30e0\u3067\u306fOpenCV\u306e\u95a2\u6570\u3067\u8f2a\u90ed\u3092\u691c\u51fa\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

cv2.findContours(img_cnt1, cv2.RETR_EXTERNAL, cv2.CHAIN_APPROX_SIMPLE)<\/p>\n

\u4e8c\u5024\u5316\u306e\u95be\u5024\u306e\u521d\u671f\u5024\u306f\u3001\u5e73\u5747\u5024\uff0b\u6a19\u6e96\u504f\u5dee\u306e\uff12\u500d\u7a0b\u5ea6\u306b\u8a2d\u5b9a\u3057\u3066\u3042\u308a\u307e\u3059\u3002<\/p>\n

threshold = int(new_img.mean() + (np.std(new_img) * 2) )
\ncv2.threshold(img_cnt1, threshold, 255, cv2.THRESH_BINARY)<\/p>\n

\u30aa\u30fc\u30d7\u30cb\u30f3\u30b0\u3001\u30af\u30ed\u30fc\u30b8\u30f3\u30b0\u51e6\u7406\u3067\u30ce\u30a4\u30ba\u9664\u53bb\u3092\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

cv2.morphologyEx(img_cnt1, cv2.MORPH_OPEN, None, iterations=iterations_open)
\ncv2.morphologyEx(img_cnt1, cv2.MORPH_CLOSE, None, iterations=iterations_close)<\/p>\n

\u30ab\u30a6\u30f3\u30c8\u3067\u304d\u308b\u9818\u57df\u306e\u9762\u7a4d\u306e\u6700\u5927\u6700\u5c0f\u3092\u8a2d\u5b9a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

list(filter(lambda x: cv2.contourArea(x) > cnt_area_small, contours))
\nlist(filter(lambda x: cv2.contourArea(x) < cnt_area_large, contours))<\/p>\n

\u30ad\u30fc\u64cd\u4f5c\u306f\u4ee5\u4e0b\u306e\u3088\u3046\u306b\u9069\u5f53\u306b\u5272\u308a\u632f\u308a\u307e\u3057\u305f\u3002
\n\u30fb\u4e8c\u5024\u5316\u306e\u95be\u5024\u306f ‘+’ ‘-‘ \u30ad\u30fc\u3067\u4e0a\u4e0b
\n\u30fb\u30aa\u30fc\u30d7\u30cb\u30f3\u30b0\u306f ‘o’ = \u5897\u52a0\u3001\u30af\u30ed\u30fc\u30b8\u30f3\u30b0 ‘c’ = \u5897\u52a0
\n\u30fb\u9818\u57df\u30b5\u30a4\u30ba ‘l’ = \u6700\u5927\u5024\u3092\u4e0b\u3052\u308b ‘s’ = \u6700\u5c0f\u5024\u3092\u4e0a\u3052\u308b
\n\u30fbEsc\u30ad\u30fc\u3067\u3084\u308a\u76f4\u3057
\n\u30fbEnter\u30ad\u30fc\u3067\u7d42\u4e86<\/p>\n

\u30ab\u30a6\u30f3\u30c8\u5024\u306f\u30b3\u30f3\u30bd\u30fc\u30eb\u306b\u8868\u793a\u3055\u308c\u307e\u3059\u3002<\/p>\n

count=130<\/p>\n

\u76ee\u8996\u3067\u6570\u3048\u305f\u5834\u5408\u306f127\u3060\u3063\u305f\u306e\u3067\u6709\u52b9\u6570\u5024\uff12\u6841\u3068\u8003\u3048\u308b\u3068\u307e\u3042\u307e\u3042\u306a\u7d50\u679c\u3068\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n

\u4e8c\u5024\u5316\u306e\u753b\u50cf\u3068\u30ab\u30a6\u30f3\u30c8\u4ed8\u304d\u306e\u5143\u753b\u50cf\u306f cntiamge1.jpg, cntiamge2.jpg \u3068\u3057\u3066\u4fdd\u5b58\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u65e2\u5b58\u306e\u8f2a\u90ed\u691c\u51fa\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u3092\u5229\u7528\u3057\u305f\u7c21\u5358\u306a\u30b5\u30f3\u30d7\u30eb\u30d7\u30ed\u30b0\u30e9\u30e0\u3067\u3059\u304c\u3001\u3042\u308b\u7a0b\u5ea6\u306e\u6210\u679c\u306f\u3042\u3052\u3089\u308c\u3066\u3044\u308b\u3068\u601d\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u57f9\u5730\u306b\u6620\u3063\u305f\u5f71\u30fb\u53cd\u5c04\u5149\u306a\u3069\u306e\u554f\u984c\u3084\u57f9\u5730\u306e\u6df5\u30fb\u30b3\u30ed\u30cb\u30fc\u304c\u4e00\u90e8\u91cd\u306a\u3063\u3066\u3044\u308b\u5834\u5408\u306a\u3069\u6b63\u78ba\u306b\u30ab\u30a6\u30f3\u30c8\u3067\u304d\u3066\u3044\u306a\u3044\u90e8\u5206\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u30c7\u30a3\u30fc\u30d7\u30e9\u30fc\u30cb\u30f3\u30b0\u306b\u3088\u308a\u89e3\u6c7a\u3059\u308b\u3079\u304d\u8ab2\u984c<\/p>\n

\u30fb\u89e3\u50cf\u5ea6\u30fb\u660e\u308b\u3055\u30fb\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u306e\u8abf\u6574<\/p>\n

\u30fb\u5149\u306e\u53cd\u5c04\u30fb\u5f71\u306a\u3069\u306e\u8abf\u6574<\/p>\n

\u30fb\u6ce1\u3001\u30b4\u30df\u306e\u3088\u3046\u306a\u4f59\u8a08\u306a\u3082\u306e\u3092\u6d88\u53bb<\/p>\n

\u30fb\u30b3\u30ed\u30cb\u30fc\u306e\u5927\u304d\u3055\u3084\u8272\u306e\u8abf\u6574<\/p>\n

\u30fb\u30b3\u30ed\u30cb\u30fc\u306e\u91cd\u306a\u308a\u306e\u88dc\u6b63<\/p>\n

\u3053\u308c\u3089\u306b\u3064\u3044\u3066\u306f\u300ctensorflow \u81ea\u52d5\u7740\u8272 + OpenCV \u8f2a\u90ed\u62bd\u51fa\u3067\u82b1\u306e\u6570\u3092\u30ab\u30a6\u30f3\u30c8<\/a>\u300d\u3084\u300cTensorFlow + OpenCV \u690d\u7269\u5199\u771f\u306e\u30de\u30b9\u30ad\u30f3\u30b0<\/a>\u300d\u306e\u3088\u3046\u306a\u8003\u3048\u65b9\u3067\u5fdc\u7528\u304d\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u307e\u305f\u3001\u57f9\u5730\u306e\u7bc4\u56f2\u306e\u8a2d\u5b9a\u306b\u3064\u3044\u3066\u306f\u3001\u57f9\u5730\u3092\u8a8d\u8b58\u3057\u3066\u81ea\u52d5\u691c\u51fa\u3059\u308b\u304b\u3001\u57f9\u5730\u306e\u67a0\u3092\u6d88\u3059\u306a\u3069\u306e\u5b66\u7fd2\u65b9\u6cd5\u304c\u8003\u3048\u3089\u308c\u308b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\uff082022\/06\/07 Shin Onda\uff09<\/p>\n","protected":false},"excerpt":{"rendered":"\uff08\u300c\uff21\uff29\u8ab2\u984c\uff12\uff1a\uff21\uff29\u3067\u751f\u83cc\u6570\u3092\u30ab\u30a6\u30f3\u30c8\uff08\uff11\uff09\u300d\u306e\u7d9a\u304d\u3067\u3059\u3002\uff09 \u307e\u305a\u6bd4\u8f03\u7684\u7c21\u5358\u306b\u3067\u304d\u305d\u3046\u306a\u300c(2) \u65e2\u5b58\u306e\u81ea\u52d5\u30ab\u30a6\u30f3\u30bf\u306e\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u306e\u524d\u51e6\u7406\u3068\u3057\u3066\u30c7\u30a3\u30fc\u30d7\u30e9\u30fc\u30cb\u30f3\u30b0\u306e\u5b66\u7fd2\u7d50\u679c\u3092\u4f7f\u3046\u5834\u5408\u300d\u306b\u3064\u3044\u3066\u8003\u3048\u308b\u3053\u3068\u306b\u3057\u307e\u3059\u3002 \u30c7\u30a3\u30fc …","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-1498","post","type-post","status-publish","format-standard","hentry","category-ai_ecobio"],"_links":{"self":[{"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=\/wp\/v2\/posts\/1498","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1498"}],"version-history":[{"count":6,"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=\/wp\/v2\/posts\/1498\/revisions"}],"predecessor-version":[{"id":1517,"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=\/wp\/v2\/posts\/1498\/revisions\/1517"}],"wp:attachment":[{"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1498"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1498"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.ecobioinfo.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1498"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}